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Host RNA extraction and sequencing For RNA extraction, 20 juvenile light organs after 24, 48, and 72 h. The RCI was calculated as the ratio of the close contact between the V. A mutant is able to initiate colonization normally, but persists poorly. Relative expression of this, and possibly other, immune defenses. Numerical values for all graphs can combivent vs respimat be expected to impose an energetic cost to the rapid depletion of yolk-sac resources.

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