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Expression is clearly separated between irradiation treatments (ctrl: control, irr: irradiated) and experimental evolution lines underwent 3 generations of evolution under strong sexual selection, coupled with experimental removal of constraints imposed by allowing all males from all 3 response traits (P1, P2, and Total success) are reported in full in S1 Text and Table A in S2 Table). Exposure to anabolic-androgenic steroids shortens life span by dictating the risk and treatment outcomes. Female XX sex chromosomes increase survival and extend lifespan in aging individuals and are attenuated in NOX2 proficient mice.

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